Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bio-separations often require ion-pairing reagents, such as Trifluoroacetic acid (TFA), in combination with ...

With the global surge in plastic consumption, sustainable recycling has emerged as a cornerstone of environmental stewardship ...

There are a few scenarios where analytical procedures might need to be transferred between HPLC instruments: 1) Method transfer to new equipment in the same lab, perhaps to a different vendor or a ...

Gradient elution involves a programmed change in mobile-phase composition over time, transitioning from weak to strong during a separation. Linear gradients, characterized by a constant change in ...

The authors discuss the approach taken to develop a new calibration approach, its associated protocols, and how it can be used to calculate data. The use of open-access high-performance liquid ...

When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...

High-Performance Liquid Chromatography (HPLC) is a very useful technique to separate chemical components of a mixture by passing the mixture (the liquid phase) through a column packed with some type ...