SOLVED:What is the principle behind real-time PCR?

Basic Molecular Biology: PCR and Real-Time PCR – Principle of PCR

Polymerase Chain Reaction (PCR) Explained. PCR, especially real-time PCR, has revolutionized the way clinical microbiology laboratories diagnose many human microbial infections. Real-time PCR monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly quantitative. With qPCR, fluorescent dyes are use

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